ارتباط چند ریختیG-308A ژن TNF-α با نمایه توده بدنی و فاکتورهای موثر در چاقی در مطالعه قند و لیپید تهران

  Background: obesity cause a lot of disease such as cardiovascular, type 2 diabetes, obstructive sleep apnea, some types of cancers and osteoarthritis. Reports show polymorphism at position -308 in the promoter region of TNF- α increase transcription of the gene in adipocytes. We, therefore, examined the relationship between this variant and BMI as an obesity criterion and other obesity affective factors in an Iranian population.   Methods : 244 Subjects were randomly selected from the Tehran lipid and Glucose study which is a Cross Sectional Study and they were classified in two age groups under 18 and above 18. We measured FBS, HDL-C, LDL-C, triglyceride, cholesterol levels, CRP, IL-6, IL-10, Adiponectin, and HOMA for all individuals. Factors including Body mass index and blood pressure were measured too .A 107bp segment of the mentioned gene with PCR (Polymerase Chain Reaction) was amplified and the polymorphism with RFLP (Restriction Fragment Length Polymorphism) revealed. Undistributed Data were analyzed by kruskal-Wallis test and distributed Data were analyzed by ANOVA. We used one-way ANOVA test followed by post hoc multiple comparison (Tukey) to compare laboratory findings of BMI groups . All data were analyzed using SPSS 16.   Results: The allele frequency of TNF-α polymorphism was in the Hardy Weinberg equilibrium and the Genotype of all 244 subjects were GG(85.7 % ), GA(13.1 % ),AA(1.2 % ) and there was no relation between BMI and the frequency of this allele. In adolescent sbp ) systolic blood pressure) in girls at GA state was significantly more than GG and CRP in boys at AA state was significantly more than the two other state In adult cholesterol in men at AA was significantly more than the two other state and LDL-C in women in GG was more than the two other state .   Conclusion: Data from this study shows no association between G - 308A TNF-a promoter polymorphism with increasing BMI, so probably it is not important risk factor for obesity in Iranian populations.